| Product Name | EliKine™ Rat TGF-β1 ELISA Kit |
|---|---|
| Description | EliKine™ Rat TGF-β1 ELISA Kit has high sensitivity and excellent specificity for detection of Rat TGF-β1. No significant cross-reactivity or interference between Rat TGF-β1 and analogues was observed. Transforming growth factor β (TGF β) is a pleiotropic cytokine that exhibits a broad spectrum of biological and regulatory effects on the cellular and organism level. It plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels (e.g. mRNA turnover, latent protein activation or post-translational modifications). EliKine™ Rat TGF-β1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-β1 in samples. An antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-β1 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-β1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 protein, latency-associated peptide |
| Method | Sandwich ELISA |
| Detection Range | 15.6 pg/mL-1000 pg/mL |
| Sensitivity | 8 pg/mL |
| Reactivity | Rat |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 59086 |
| Storage | 2-8°C |
| Supplier | Abbkine |
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