| Product Name | EliKine™ Human Carcinoembryonic Antigen (CEA) ELISA Kit |
|---|---|
| Description | EliKine™ Human Carcinoembryonic Antigen (CEA) ELISA Kit employs a two-site sandwich ELISA to quantitate Human CEA |
| Synonyms | Carcinoembryonic antigen ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; CD66e ELISA Kit; CEA ELISA Kit; Ceacam5 ELISA Kit; CEAM5_HUMAN ELISA Kit. |
| Detection Range | 0.625 ng/mL-40 ng/mL |
| Sensitivity | 0.313 ng/mL |
| Reactivity | Human |
| Applications | ELISA |
| Sample Types | Cell culture supernatants#Serum#Plasma |
| Reconstitution | EliKine™ Human Carcino-embryonic antigen (CEA) ELISA Kit employs a double antibody sandwich method to quantitate Human CEA in samples. An antibody specific for Human CEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human CEA present is bound by the immobilized antibody. After removing any unbound substances, a HRP-conjugated antibody specific for Human CEA is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human carcino-embryonic antigen (CEA) concentration is proportional to the OD450 nm value. |
| Storage | Stored at 4℃ for 12 months |
| Notes |
• 1. If Sample Diluent (5×) and Assay Buffer (5×) appears to turn yellow or a small amount of precipitation, etc., it is caused by the serum contained in the reagent. Please centrifuge to remove the precipitate, which will not affect normal use. • 2. Do not mix or substitute reagents with those from other lots or sources. • 3. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. • 4. To ensure accurate results, proper adhesion of plate covers during incubation steps is necessary. • 5. Stop Solution has certain Corrosive. Please take protective measures when operating. |
| Supplier | Abbkine |
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