| Product Name | EliKine™ Human Prolactin ELISA Kit |
|---|---|
| Description | EliKine™ Human Prolactin ELISA Kit has high sensitivity and excellent specificity for detection of Human Prolactin. No significant cross-reactivity or interference between Human Prolactin and analogues was observed. Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females. Prolactin is predominantly found as 199 amino acid, 25 kDa glycosylated and 23 kDa non-glycosylated monomers. Human prolactin shares only 60% and 63% amino acid sequence identity with mouse and rat prolactin, respectively, although rat prolactin can activate the human prolactin recepto. Although better characterized in rodents than humans, post-translational modifcations such as polymerization, glycosylation, and proteolytic cleavage can alter the activities of prolactin. Non-glycosylated prolactin is produced by the pituitary gland and packaged in storage granules before secretion. Glycosylated prolactin is reported to be constitutively secreted, have lower biological potency, and be removed from the circulation more quickly. EliKine™ Human Prolactin ELISA Kit employs a two-site sandwich ELISA to quantitate Prolactin in samples. An antibody specific for prolactin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any prolactin present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for prolactin is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PRL, Prl1a1, Prol, Prolactin precursor, RATPRLSD1, RNPROL, RP23-351I6.2, RP3-404K8.1, OTTMUSP00000000651, Luteotropic Hormone, Lactogenic Hormone, Mammotropin, AV290867 |
| Detection Range | 40 μIU/ml-2000 μIU/ml |
| Sensitivity | 50 μIU/ml |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids |
| Gene Id | 5617 |
| Storage | 2-8°C |
| Background | Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females. Prolactin is predominantly found as 199 amino acid, 25 kDa glycosylated and 23 kDa non-glycosylated monomers. Human prolactin shares only 60% and 63% amino acid sequence identity with mouse and rat prolactin, respectively, although rat prolactin can activate the human prolactin recepto. Although better characterized in rodents than humans, post-translational modifcations such as polymerization, glycosylation, and proteolytic cleavage can alter the activities of prolactin. Non-glycosylated prolactin is produced by the pituitary gland and packaged in storage granules before secretion. Glycosylated prolactin is reported to be constitutively secreted, have lower biological potency, and be removed from the circulation more quickly. |
| Supplier | Abbkine |
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