| Product Name | EliKine™ Mouse IL-10 ELISA Kit |
|---|---|
| Description | EliKine™ Mouse IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-10. |
| Synonyms | IL 10; IL10; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor |
| Detection Range | 93.75 pg/mL- 6000 pg/mL |
| Sensitivity | 50 pg/mL |
| Reactivity | Mouse |
| Applications | ELISA |
| Sample Types | Cell culture supernatants#Serum#Plasma#Other biological fluids |
| Reconstitution | EliKine™ Mouse IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-10 in samples. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Gene Id | 16153 |
| Storage | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Notes |
• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Supplier | Abbkine |
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