| Product Name | EliKine™ Triiodothyronine (T3) ELISA Kit |
|---|---|
| Description | EliKine™ T3 ELISA Kit employs a competitive ELISA to quantitate T3. |
| Detection Range | 0.5 ng/ml-8 ng/ml |
| Sensitivity | 0.4 ng/ml |
| Reactivity | Gallus, Monkey, Bovine, Canine, Goat, Human, Mouse, Porcine, Rabbit, Rat, Sheep |
| Applications | ELISA |
| Sample Types | Serum#Plasma#Other biological fluids |
| Reconstitution | EliKine™ T3 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured. |
| Storage | The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols. |
| Notes |
• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for |
| Supplier | Abbkine |
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