| Product Name | ELN ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Elastin (ELN). No significant cross-reactivity or interference between Elastin (ELN) and analogues was observed. |
| Synonyms | WBS, WS, SVAS; Tropoelastin; Supravalvular Aortic Stenosis; Williams-Beuren Syndrome |
| Method | Sandwich |
| Detection Range | 4.7-300ng/mL |
| Sensitivity | 1.5ng/mL |
| Target | ELN |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Elastin (ELN). No significant cross-reactivity or interference between Elastin (ELN) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P15502 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Induction of elastin expression in vascular endothelial cells relates to hepatoportal sclerosis in idiopathic portal hypertension: possible link to serum anti-endothelial cell antibodies;Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly.;A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins;Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media;Effects of Exendin-4 on human adipose tissue inflammation and ECM remodelling.;Anti-ageing effects of Ubiquinone and Ubiquinol in a senescence model of human dermal fibroblasts;GENE THERAPY DNA VECTOR VTvaf17, METHOD OF PRODUCTION; ESCHERICHIA COLI STRAIN SCS110-AF, METHOD OF PRODUCTION; ESCHERICHIA?¡;CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE; |
| Supplier | Cloud Clone |
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