Product Name | ENTPD3, 44-485aa, Human (Denatured) |
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Description | ENTPD3 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD3 contains 4 apyrase-conserved regions which is characteristic of NTPases. Recombinant human ENTPD3 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
Synonyms | Ectonucleoside triphosphate diphosphohydrolase 3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39L3, HB6, NTPDase-3 |
Host | E. coli |
Molecular Weight | 44.5 kDa (406aa) confirmed by MALDI-TOF |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSQIHKQEV LPPGLKYGIV LDAGSSRTTV YVYQWPAEKE NNTGVVSQTF KCSVKGSGIS SYGNNPQDVP RAFEECMQKV KGQVPSHLHG STPIHLGATA GMRLLRLQNE TAANEVLESI QSYFKSQPFD FRGAQIISGQ EEGVYGWITA NYLMGNFLEK NLWHMWVHPH GVETTGALDL GGASTQISFV AGEKMDLNTS DIMQVSLYGY VYTLYTHSFQ CYGRNEAEKK FLAMLLQNSP TKNHLTNPCY PRDYSISFTM GHVFDSLCTV DQRPESYNPN DVITFEGTGD PSLCKEKVAS IFDFKACHDQ ETCSFDGVYQ PKIKGPFVAF AGFYYTASAL NLSGSFSLDT FNSSTWNFCS QNWSQLPLLL PKFDEVYARS YCFSANYIYH LFVNGYKFTE ETWPQIHFEK EVGNSSIAWS LGYMLSLTNQ IPAESPLIRL PIEPP |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 20mM Phosphate buffer saline (pH 8.0) containing 30% glycerol, 1mM DTT |
Concentration | 0.25 mg/ml (determined by Bradford assay) |
Purity | > 85% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
References | Yang F., et al (2001). Biochemistry 40:3943-3950. |
Background | ENTPD3 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD3 contains 4 apyrase-conserved regions which is characteristic of NTPases. Recombinant human ENTPD3 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
Supplier | ARP |
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