| Product Name | EPHB2, 19-543aa, Human, Baculovirus |
|---|---|
| Description | EPHB2, also known as ephrin type-B receptor 2 isoform 1, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs) that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverse signaling in the ligand-expressing cells through Ephrin. Hippocampal neurons can release vesicles containing full length EPHB2, and these are taken up by neighboring glial cells. This protein controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity. Recombinant human EPHB2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | Ephrin type-B receptor 2 isoform 1, EPHB2, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5 |
| Host | Virus |
| Molecular Weight | 47.7 kDa (426aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | VEETLMDSTT ATAELGWMVH PPSGWEEVSG YDENMNTIRT YQVCNVFESS QNNWLRTKFI RRRGAHRIHV EMKFSVRDCS SIPSVPGSCK ETFNLYYYEA DFDSATKTFP NWMENPWVKV DTIAADESFS QVDLGGRVMK INTEVRSFGP VSRSGFYLAF QDYGGCMSLI AVRVFYRKCP RIIQNGAIFQ ETLSGAESTS LVAARGSCIA NAEEVDVPIK LYCNGDGEWL VPIGRCMCKA GFEAVENGTV CRGCPSGTFK ANQGDEACTH CPINSRTTSE GATNCVCRNG YYRADLDPLD MPCTTIPSAP QAVISSVNET SLMLEWTPPR DSGGREDLVY NIICKSCGSG RGACTRCGDN VQYAPRQLGL TEPRIYISDL LAHTQYTFEI QAVNGVTDQS PFSPQFASVN ITTNQAAPSA VSIMHQVSRT VDSITLSWSQ PDQPNGVILD YELQYYEKEL SEYNATAIKS PTNTVTVQGL KAGAIYVFQV RARTVAGYGR YSGKMYFQTM TEAEYQTSIQ EKLPLLEHHH HHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Pasquale EB. (2008) Cell. 133:38-52. Cowan CA., et al. (2000) Neuron 26:417-430. |
| Background | EPHB2, also known as ephrin type-B receptor 2 isoform 1, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs) that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverse signaling in the ligand-expressing cells through Ephrin. Hippocampal neurons can release vesicles containing full length EPHB2, and these are taken up by neighboring glial cells. This protein controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity. Recombinant human EPHB2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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