EPI ELISA kit (General species)

Category: Assay Kits
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CEA858Ge
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Product Name EPI ELISA kit (General species)
Description This assay has high sensitivity and excellent specificity for detection of Epinephrine (EPI). No significant cross-reactivity or interference between Epinephrine (EPI) and analogues was observed.
Synonyms Adrenaline
Method Competitive
Detection Range 24.69-2,000pg/mL
Sensitivity 9.74pg/mL
Target EPI
Specificity This assay has high sensitivity and excellent specificity for detection of Epinephrine (EPI). No significant cross-reactivity or interference between Epinephrine (EPI) and analogues was observed.
Sample Types Serum, Plasma, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References METHODS AND REAGENTS FOR THE DIAGNOSIS, PREVENTION AND TREATMENT OF INSULIN RESISTANCE;Glibenclamide attenuates myocardial injury by lipopolysaccharides in streptozotocin-induced diabetic mice;Study of baicalin on sympathoexcitation induced by myocardial ischemia via P2X3 receptor in superior cervical ganglia;Catha edulis chewing effects on treatment of paranoid schizophrenic patients;Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria;Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity;Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial;Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance;RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis;Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis;Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair …;Anti-stress activity of some plants extracts of the North Caucasus flora;Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D;O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress;Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy …;
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