Erythropoietin, 28-193aa, Human, Baculovirus (Bioactivity Validated)
| Product Name | Erythropoietin, 28-193aa, Human, Baculovirus (Bioactivity Validated) |
|---|---|
| Description | EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant human EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | EPO, EP, MVCD2 |
| Host | Virus |
| Molecular Weight |
24.1kDa (217aa) 28-40KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDRLEHH HHHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.5mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Koury, M.J., et al. (2005) Exp. Hematol. 33:1263. Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205 |
| Background | EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant human EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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