ESM1 ELISA kit (Human)

Category: Assay Kits
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SEC463Hu
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Product Name ESM1 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Endothelial Cell Specific Molecule 1 (ESM1). No significant cross-reactivity or interference between Endothelial Cell Specific Molecule 1 (ESM1) and analogues was observed.
Synonyms Endocan
Method Sandwich
Detection Range 0.156-10ng/mL
Sensitivity 0.065ng/mL
Target ESM1
Specificity This assay has high sensitivity and excellent specificity for detection of Endothelial Cell Specific Molecule 1 (ESM1). No significant cross-reactivity or interference between Endothelial Cell Specific Molecule 1 (ESM1) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot Q9NQ30
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Detection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantation;Endocan is useful biomarker of survival and severity in sepsis.;Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes;Endocan is useful biomarker of survival and severity in sepsis;Endocan Levels and Subclinical Atherosclerosis in Patients With Systemic Lupus Erythematosus;Assessment of subclinical cardiac damage in chronic plaque psoriasis patients: a case control study;Diagnostic and prognostic values of endothelial-cell-specific molecule-1 with malignant pleural effusions in patients with non-small cell lung cancer;Changes in Endocan Levels and Blood Coagulation in HIV Infection;Is the plasma endocan level a reliable predictor for the severity of erectile dysfunction?;Serum Level of Endothelial Cell-Specific Molecule-1 (ESM-1) as a New Potential Biomarker for Rheumatoid Arthritis Disease Activity;Endothelial biomarkers in the light of new sepsis definition;Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with …;A novel marker relationship between carotid intima–media thickness and disease activity score-28 in patients with rheumatoid arthritis: human endothelial cell …;Role of Ascitic Endocan Levels in the Diagnosis of Spontaneous Bacterial Peritonitis in Decompensated Cirrhosis;Comparison of the maternal serum endocan levels in preterm premature rupture of membrane and normal pregnancy;The relationship between serum endocan levels with the presence of contrast-induced nephropathy in patients undergoing coronary angiography;
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