| Product Name | ExKine™ Membrane and Cytoplasmic Protein Extraction kit |
|---|---|
| Description | Abbkine ExKine™ Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of cytoplasmic and membrane protein. |
| Reconstitution | Abbkine ExKine™ Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of non-denatured, active cytoplasmic and membrane protein from mammalian cultured cells or tissues, which can be used directly in a variety of proteomics application. The cells are first broken by homogenization to allow the release of cytosolic and membrane proteins, after which membrane proteins are obtained by high speed centrifugation. Extraction efficiencies and yields will vary depending on cell type as well as the number of times the integral membrane protein spans the lipid bilayer. |
| Storage | Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice. |
| Notes | Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous. |
| Supplier | Abbkine |
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