| Product Name | ExKine™ Total Membrane Protein Extraction Kit |
|---|---|
| Description | Abbkine ExKine™ Total Membrane Protein Extraction Kit provides a simple, rapid and reproducible method to extract total membrane proteins. |
| Reconstitution | Abbkine ExKine™ Total Membrane Protein Extraction Kit is a simple, rapid and reproducible method to prepare cellular protein fractions highly enriched in membrane and hydrophobic proteins, based on temperature-dependent phase separation. The sample is first mixed and homogenized in the membrane protein extraction buffers. After a brief incubation at 37°C, the sample is centrifuged, producing an upper aqueous phase and a lower detergent-rich phase. Proteins that were anchored to the membrane or proteins containing one or two transmembrane domains are efficiently partitioned to the detergent-rich phase. |
| Storage | Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice. |
| Notes | Ensure all reagents and solutions are at the appropriate temperature before starting the assay. |
| Supplier | Abbkine |
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