FABP4 ELISA kit (Rat)

Category: Assay Kits
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SEB693Ra
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Product Name FABP4 ELISA kit (Rat)
Description This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 4, Adipocyte (FABP4). No significant cross-reactivity or interference between Fatty Acid Binding Protein 4, Adipocyte (FABP4) and analogues was observed.
Synonyms A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein
Method Sandwich
Detection Range 1.56-100ng/mL
Sensitivity 0.60ng/mL
Target FABP4
Specificity This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 4 (FABP4). No significant cross-reactivity or interference between Fatty Acid Binding Protein 4 (FABP4) and analogues was observed.
Reactivity Rat
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P70623
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …;Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …;
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