FBLN3 ELISA kit (Human)

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SEF422Hu
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Product Name FBLN3 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Fibulin 3 (FBLN3). No significant cross-reactivity or interference between Fibulin 3 (FBLN3) and analogues was observed.
Synonyms EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; Fibrillin-Like; Extracellular protein S1-5
Method Sandwich
Detection Range 1.56-100ng/mL
Sensitivity 0.64ng/mL
Target FBLN3
Specificity This assay has high sensitivity and excellent specificity for detection of Fibulin 3 (FBLN3). No significant cross-reactivity or interference between Fibulin 3 (FBLN3) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Urine And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot Q12805
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Fibulin-3 as a Blood and Effusion Biomarker for Pleural Mesothelioma;YKL-40 and mesothelin in the blood of patients with malignant mesothelioma, lung cancer and asbestosis.;Role of fibulin-3 in lung cancer: In vivo and in vitro analyses;Discovery of new biomarkers for malignant mesothelioma;DIAGNOSIS OF CELL PROLIFERATIVE DISEASES;Comparison of fibulin-3 and mesothelin as markers in malignant mesothelioma;Value of plasma and pleural effusion fibulin-3 levels in the diagnosis of malignant pleural mesothelioma effusions;Fibulin-3 as a biomarker of response to treatment in malignant mesothelioma;Fibulin-3 levels in malignant pleural mesothelioma are associated with prognosis but not diagnosis;Plasma Biomarker Enrichment of Clinical Prognostic Indices in Malignant Pleural Mesothelioma.;Diagnostic and Prognostic Biomarkers in the Rational Assessment of Mesothelioma (DIAPHRAGM) study: protocol of a prospective, multicentre, observational study ;HMGB1 and its hyperacetylated isoform are sensitive and specific serum biomarkers to detect asbestos exposure and to identify mesothelioma patients;Diagnostic and Prognostic Biomarkers in the Rational Assessment of Mesothelioma (DIAPHRAGM) study: protocol of a prospective, multicentre, observational …;Comparison of the Diagnostic Performance of Fibulin-3 and Mesothelin in Patients with Pleural Effusions from Malignant Mesothelioma.;A Novel Panel of Serum Biomarkers for MPM Diagnosis.;Association of fibulin-3 concentrations with the presence and severity of knee osteoarthritis: A cross-sectional study;Plasma Fibulin-3 as a Potential Biomarker for Patients with Asbestos-Related Diseases in the Han Population;Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes;The matrix protein Fibulin-3 promotes KISS1R induced triple negative breast cancer cell invasion;Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with …;Prediction of pneumoconiosis by serum and urinary biomarkers in workers exposed to asbestos-contaminated minerals;
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