| Product Name | Fish Dehydroepiandrosterone (DHEA) ELISA Kit |
|---|---|
| Description | Fish Dehydroepiandrosterone (DHEA) ELISA Kit has high sensitivity and excellent specificity for detection of FishDHEA. No significant cross-reactivity or interference between FishDHEA and analogues was observed. Dehydroepiandrosterone (DHEA), also known as androstenolone or as prasterone (INN), is an endogenous steroid hormone. It is one of the most abundant circulating steroids in humans, in whom it is produced in the adrenal glands, the gonads, and the brain, where it functions as a metabolic intermediate in the biosynthesis of the androgen and estrogen sex steroids. However, DHEA also has a variety of potential biological effects in its own right, binding to an array of nuclear and cell surface receptors, and acting as a neurosteroid and neurotrophin. DHEA is also used orally as a medication and dietary supplement, to restore or increase DHEA and DHEA sulfate (DHEA-S) levels. This Fish Dehydroepiandrosterone (DHEA) ELISA Kit employs a two-site sandwich ELISA to quantitate DHEA in samples. An antibody specific for DHEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHEA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHEA bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DHEA, DHEA-ST, DHEAS, HST, ST2, ST2A3, STD, hSTa, alcohol sulfotransferase, alcohol/hydroxysteroid sulfotransferase, bile-salt sulfotranasferase 2A1, bile-salt sulfotransferase 2A1, dehydroepiandrosterone sul |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Fish |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Supplier | Abbkine |
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