| Product Name | Fish Interleukin 1α (IL- 1A) ELISA Kit |
|---|---|
| Description | Fish Interleukin 1α (IL- 1A) ELISA Kit has high sensitivity and excellent specificity for detection of FishIL- 1A. No significant cross-reactivity or interference between FishIL- 1A and analogues was observed. IL-1α is a member of interleukin 1 family. IL-1α and IL-1β recognize the same IL-1 receptor and share a number of similar biological functions. IL-1α is predominantly a cell-associated molecule whereas IL-1β is a secreted molecule.IL-1α is synthesized primarily as a 31 kDa precursor that lacks a signal peptide. Cleavage of the precursor is via the cysteine protease calpain, resulting in a 17.5 kDa mature IL-1 molecule. Being active in the processed form, the IL-1 precursor is also biologically active via specific cell binding. A portion of the precursor is transported to the cell surface and associated with the cell membrane. Precursor IL-1α can be released and cleaved by extracellular proteases when the cells die, and can also be cleaved by activation of the calcium-dependent, membrane-associated calpains. This Fish Interleukin 1α (IL- 1A) ELISA Kit employs a two-site sandwich ELISA to quantitate IL- 1A in samples. An antibody specific for IL- 1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL- 1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL- 1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL- 1A bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | IL- 1A |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Fish |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | IL-1α is a member of interleukin 1 family. IL-1α and IL-1β recognize the same IL-1 receptor and share a number of similar biological functions. IL-1α is predominantly a cell-associated molecule whereas IL-1β is a secreted molecule.IL-1α is synthesized primarily as a 31 kDa precursor that lacks a signal peptide. Cleavage of the precursor is via the cysteine protease calpain, resulting in a 17.5 kDa mature IL-1 molecule. Being active in the processed form, the IL-1 precursor is also biologically active via specific cell binding. A portion of the precursor is transported to the cell surface and associated with the cell membrane. Precursor IL-1α can be released and cleaved by extracellular proteases when the cells die, and can also be cleaved by activation of the calcium-dependent, membrane-associated calpains. |
| Supplier | Abbkine |
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