| Product Name | Fish Na+/K+-ATPase (Na/K ATPase) ELISA Kit |
|---|---|
| Description | Sodium/potassium-transporting ATPase subunit beta-3 belongs to the family of Na /K and H /K ATPases beta chain proteins, and to the subfamily of Na+/K+-ATPases. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Fish Na+/K+-ATPase (Na/K ATPase) ELISA Kit employs a two-site sandwich ELISA to quantitate Na/K ATPase in samples. An antibody specific for Na/K ATPase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Na/K ATPase present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Fish Na/K ATPase detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Na/K ATPase bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | Na/K ATPa |
| Method | Sandwich ELISA |
| Detection Range | 0.75 μmol/L-12 μmol/L |
| Sensitivity | 0.05 μmol/L |
| Reactivity | Fish |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | Sodium/potassium-transporting ATPase subunit beta-3 belongs to the family of Na /K and H /K ATPases beta chain proteins, and to the subfamily of Na+/K+-ATPases. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. |
| Supplier | Abbkine |
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