| Product Name | FNDC5 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Fibronectin Type III Domain Containing Protein 5 (FNDC5). No significant cross-reactivity or interference between Fibronectin Type III Domain Containing Protein 5 (FNDC5) and analogues was observed. |
| Synonyms | FRCP2; Irisin; Fibronectin type III repeat-containing protein 2 |
| Method | Sandwich |
| Detection Range | 15.6-1,000pg/mL |
| Sensitivity | 6.1pg/mL |
| Target | FNDC5 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Fibronectin Type III Domain Containing Protein 5 (FNDC5). No significant cross-reactivity or interference between Fibronectin Type III Domain Containing Protein 5 (FNDC5) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | Q8NAU1 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Serum irisin levels in new-onset type 2 diabetes;Lower circulating irisin is associated with type 2 diabetes mellitus;Inconsistency in circulating irisin levels: what is really happening;Leptin administration activates irisin-induced myogenesis via nitric oxide-dependent mechanisms, but reduces its effect on subcutaneous fat browning in mice;Lower irisin level in patients with type 2 diabetes mellitus: A case-control study and meta-analysis;Maternal circulating levels of irisin in intrahepatic cholestasis of pregnancy;Lower Circulating Irisin Level in Patients with Diabetes Mellitus: A Systematic Review and Meta-Analysis;Physiology and role of irisin in glucose homeostasis.;Irisin stimulates cell proliferation and invasion by targeting the PI3K/AKT pathway in human hepatocellular carcinoma ;The Diagnostic Value of FNDC5/Irisin in Renal Cell Cancer.;Low serum irisin concentration is associated with poor outcomes in patients with acute pancreatitis and irisin administration protects against experimental acute …;Maternal serum, placental, and umbilical venous blood irisin levels in intrahepatic cholestasis of pregnancy;Serum Irisin Predicts Posthepatectomy Complications in Patients with Hepatocellular Carcinoma;The myokine Irisin: localization and effects in swine late medium and large antral ovarian follicle;Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients;Pattern of Adiponectin, Osteocalcin, Irisin, FGF-21, and MCP-1 According to the Body Size Phenotype: Could They Be Markers of Metabolic Health in Mexican-Mestizo …;Eight-week exercise program improved the quality of life of Alzheimer's patients through functional, cognitive, and biochemical parameters; |
| Supplier | Cloud Clone |
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