| Product Name | FNDC5 ELISA kit (Mouse) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Fibronectin Type III Domain Containing Protein 5 (FNDC5). No significant cross-reactivity or interference between Fibronectin Type III Domain Containing Protein 5 (FNDC5) and analogues was observed. |
| Synonyms | FRCP2; Irisin; Fibronectin type III repeat-containing protein 2 |
| Method | Sandwich |
| Detection Range | 15.6-1,000pg/mL |
| Sensitivity | 6.5pg/mL |
| Target | FNDC5 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Fibronectin Type III Domain Containing Protein 5 (FNDC5). No significant cross-reactivity or interference between Fibronectin Type III Domain Containing Protein 5 (FNDC5) and analogues was observed. |
| Reactivity | Mouse |
| Sample Types | Serum, Plasma And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | Q8K4Z2 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Leptin administration activates irisin-induced myogenesis via nitric oxide-dependent mechanisms, but reduces its effect on subcutaneous fat browning in mice;Fenofibrate (PPARalpha agonist) induces beige cell formation in subcutaneous white adipose tissue from diet-induced male obese mice;Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice;Differential actions of PPAR-α and PPAR-β/δ on beige adipocyte formation: A study in the subcutaneous white adipose tissue of obese male mice;Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative …;Identification of irisin as a therapeutic agent that inhibits oxidative stress and fibrosis in a murine model of chronic pancreatitis;Time-restricted feeding prevents metabolic diseases through the regulation of galanin/GALR1 expression in the hypothalamus of mice; |
| Supplier | Cloud Clone |
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