| Product Name | FuCA2, 29-467aa, Human (Denatured) |
|---|---|
| Description | FuCA2 is a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. This protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. FuCA2 is essential for Helicobacter pylori adhesion to human gastric cancer cells. Recombinant human FuCA2 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Plasma alpha-L-fucosidase, Plasma alpha-L-fucosidase, Fucosidase, alpha-L- 2, plasma, dJ20N2.5, RP1-20N2.5 |
| Host | E. coli |
| Molecular Weight | 24.6kDa (224aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHSATRFD PTWESLDARQ LPAWFDQAKF GIFIHWGVFS VPSFGSEWFW WYWQKEKIPK YVEFMKDNYP PSFKYEDFGP LFTAKFFNAN QWADIFQASG AKYIVLTSKH HEGFTLWGSE YSWNWNAIDE GPKRDIVKEL EVAIRNRTDL RFGLYYSLFE WFHPLFLEDE SSSFHKRQFP VSKTLPELYE LVNNYQPEVL WSDGDGGAPD QYWNSTGFLA WLYNESPVRG TVVTNDRWGA GSICKHGGFY TCSDRYNPGH LLPHKWENCM TIDKLSWGYR REAGISDYLT IEELVKQLVE TVSCGGNLLM NIGPTLDGTI SVVFEERLRQ MGSWLKVNGE AIYETHTWRS QNDTVTPDVW YTSKPKEKLV YAIFLKWPTS GQLFLGHPKA ILGATEVKLL GHGQPLNWIS LEQNGIMVEL PQLTIHQMPC KWGWALALTN VI |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in PBS buffer (pH 7.4) containing 10% glycerol, 1mM DTT |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Clark H.F., et al. (2003) Genome Re., 13:2265-2270 |
| Background | FuCA2 is a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. This protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. FuCA2 is essential for Helicobacter pylori adhesion to human gastric cancer cells. Recombinant human FuCA2 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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