| Product Name | Gankyrin, 1-226aa, Human, Recombinant |
|---|---|
| Description | Gankyrin is an oncoprotein commonly overexpressed in most hepatocellular carcinomas. Gankyrin interacts with S6 ATPase of the 19S regulatory particle of the 26S proteasome. It is known to play a role in the regulation of the phosphorylation of the retinoblastoma protein by CDK4, and to enhance the ubiquitinylation of p53 by MDM2. Gankyrin consists of 7 ankyrin repeats and is structurally similar to I kappa Bs. Recombinant human Gankyrin was expressed in E.coli and purified by conventional chromatography techniques. |
| Synonyms | proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1, ankyrin repeat protein p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, PSMD10. |
| Host | E. coli |
| Molecular Weight | 20kDa (175aa) |
| Amino Acid Sequence | MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl (pH 7.5) |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Umemura A., et al. (2008) Hepatology. 47(2):493-502. Chen Y., et al. (2007) Cell Res. 17(12): 1020-9 |
| Background | Gankyrin is an oncoprotein commonly overexpressed in most hepatocellular carcinomas. Gankyrin interacts with S6 ATPase of the 19S regulatory particle of the 26S proteasome. It is known to play a role in the regulation of the phosphorylation of the retinoblastoma protein by CDK4, and to enhance the ubiquitinylation of p53 by MDM2. Gankyrin consists of 7 ankyrin repeats and is structurally similar to I kappa Bs. Recombinant human Gankyrin was expressed in E.coli and purified by conventional chromatography techniques. |
| Supplier | ARP |
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