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|Product Name||glpE, 1-108aa|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A|
|Background||Thiosulfate:cyanide sulfurtransferase (rhodanese), also known as glpE, is a mitochondrial matrix enzyme that is encoded by the nucleus. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily. Recombinant E.coli glpE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol|
|Intended Use||For Research Use Only|
|Molecular Weight||39.5kDa (340aa)|
|NCBI Accession #||NP_417883|
|Purity||> 95% by SDS-PAGE|
|References||Li H., et al. (2011) Biomol NMR Assign. 5(1):97-9
Ray WK., et al. (2000) J Bacteriol. 182(8):2277-84.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Thiosulfate:cyanide sulfurtransferase (rhodanese), ECK3411, JW3388|
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