| Product Name | Goat anti-14-3-3 sigma / Stratifin Antibody |
|---|---|
| Synonyms | SFN; 14-3-3sigma; stratifin; HGNC:10773; YWHAS |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-DLHTLSEDSYKDST |
| Molecular Weight | 27.8 |
| Target | 14-3-3 sigma / Stratifin |
| Amino Acid Sequence | DLHTLSEDSYKDST |
| Reactivity | Canine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:16000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 26-28kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1). Recommended concentration: 0.03-0.1µg/ml. P. IHC: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4µg/ml. |
| Gene Id | Human GeneIDs: 2810. Non-Human GeneIDs: 55948 (mouse); 313017 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Lodygin D, Hermeking H., The role of epigenetic inactivation of 14-3-3sigma in human cancer., Cell Res. 2005 Apr;15(4):237-46. Review., PMID: 15857578 |
| Supplier | Everest Biotech |
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