Goat anti-ACPP / PAP Antibody

Category: Antibodies
Catalog
EB09390
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Product Name Goat anti-ACPP / PAP Antibody
Synonyms ACPP; acid phosphatase, prostate; ACP-3; ACP3; PAP; prostatic acid phosphotase
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-YGIHKQKEKSRLQ
Molecular Weight 44.6
Target ACPP / PAP
Amino Acid Sequence YGIHKQKEKSRLQ
Reactivity Bovine, Canine, Human, Mouse, Rat
Applications ELISA, IF, IHC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:64000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 50kDa band observed in Human Prostate lysates (calculated MW of 44.6kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 Oct;32(10):1832-. IHC: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5µg/ml. This antibody has been successfully used in IHC on Mouse: Nousiainen HO et al. (2014) PMID: 24846136.. Notes: Immunofluorescence:: This antibody has been successfully used in IF on Mouse: Nousiainen HO et al. (2014) PMID: 24846136.
Gene Id Human GeneIDs: 55. Non-Human GeneIDs: 56318 (mouse); 56780 (rat);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Quintero IB, Araujo CL, Pulkka AE, Wirkkala RS, Herrala AM, Eskelinen EL, Jokitalo E, Hellström PA, Tuominen HJ, Hirvikoski PP, Vihko PT., Prostatic acid phosphatase is not a prostate specific target., Cancer research 2007 Jul 67 (14): 6549-54., PMID: 17638863
Supplier Everest Biotech

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