| Product Name | Goat anti-AIFM1 (aa183-195) Antibody |
|---|---|
| Synonyms | AIFM1; apoptosis-inducing factor, mitochondrion-associated, 1; AIF; CMTX4; COWCK; COXPD6; PDCD8; apoptosis-inducing factor 1, mitochondrial; programmed cell death 8 (apoptosis-inducing factor); striatal apoptosis-inducing factor |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-DDPNVTKTLRFKQ |
| Molecular Weight | 66.9; 66.3; 35.6; 28.4; 35.4 |
| Target | AIFM1 (aa183-195) |
| Amino Acid Sequence | DDPNVTKTLRFKQ |
| Reactivity | Bovine, Human |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9kDa according to NP_004199.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5-7µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and NIH3T3 cells. Recommended concentration: 5-10µg/ml. |
| Gene Id | Human GeneIDs: 9131 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Lewis EM, Wilkinson AS, Jackson JS, Mehra R, Varambally S, Chinnaiyan AM, Wilkinson JC., The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells., The Journal of biological chemistry 2012 Dec 287 (52): 43862-75., PMID: 23118229 |
| Supplier | Everest Biotech |
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