Goat anti-ALOX15 Antibody

Category: Antibodies
Catalog
EB09049
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Product Name Goat anti-ALOX15 Antibody
Synonyms ALOX15; arachidonate 15-lipoxygenase; 15-LOX-1; 15-LOX2
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-HYKTDVAVKDDPE
Molecular Weight 74.8
Target ALOX15
Amino Acid Sequence HYKTDVAVKDDPE
Reactivity Human
Applications ELISA, IHC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2µg/ml.
Gene Id Human GeneIDs: 246
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL., Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes., Diabetes. 2008 Jan;57(1):199-208. Epub 2007 Oct 16., PMID: 17940120
Supplier Everest Biotech

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