| Product Name | Goat anti-Androgen Receptor Antibody |
|---|---|
| Synonyms | AR; androgen receptor; dihydrotestosterone receptor; KD; AIS; TFM; DHTR; SBMA; NR3C4; SMAX1; HUMARA; HYSP1; androgen receptor; dihydrotestosterone receptor; RP11-383C12.1 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | EVQLGLGRVYPRPPSC |
| Molecular Weight | 99.2 |
| Target | Androgen Receptor |
| Amino Acid Sequence | EVQLGLGRVYPRPPSC |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Applications | ChIP, ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. P. IHC: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10µg/ml. Chromatin Immunoprecipitation: A six-fold increased presence of the F |
| Gene Id | Human GeneIDs: 367. Non-Human GeneIDs: 11835 (mouse); 24208 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Trapman J, Klaassen P, Kuiper GG, van der Korput JA, Faber PW, van Rooij HC, Geurts van Kessel A, Voorhorst MM, Mulder E, Brinkmann AO. , Cloning, structure and expression of a cDNA encoding the human androgen receptor., Biochem Biophys Res Commun. 1988 May 31;153(1):241-8. , PMID: 3377788 |
| Supplier | Everest Biotech |
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