Goat anti-AREB6 / ZEB1 Antibody

Category: Antibodies
Catalog
EB09166
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Product Name Goat anti-AREB6 / ZEB1 Antibody
Synonyms ZEB1; AREB6; zinc finger E-box binding homeobox 1; BZP; DELTA-EF1; MGC133261; NIL-2-A; NIL-2A; NIL2A; TCF8; ZEB; ZFHEP; ZFHX1A; OTTHUMP00000019405; transcription factor 8 (represses interleukin 2 expression); zinc finger homeodomain enhancer-binding prote
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-RPQIRQKIENKPLQE
Molecular Weight 122; 124
Target AREB6 / ZEB1
Amino Acid Sequence RPQIRQKIENKPLQE
Reactivity Human, Mouse, Rat
Applications ELISA, IF, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx.100kDa band observed in Mouse Heart lysates (calculated MW of 122kDa according to Human NP_110378.3 and Mouse NP_035676.1). Recommended concentration: 0.05-0.2µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human and R. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.
Gene Id Human GeneIDs: 6935. Non-Human GeneIDs: 21417 (mouse); 25705 (rat);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Park SM, Gaur AB, Lengyel E, Peter ME., The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2., Genes Dev. 2008 Apr 22 (7): 894-907., PMID: 18381893
Supplier Everest Biotech

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