| Product Name | Goat anti-ARPC2 Antibody |
|---|---|
| Synonyms | ARPC2; actin related protein 2/3 complex, subunit 2, 34kDa; ARC34; PRO2446; p34-Arc; PNAS-139; ARP2/3 protein compex subunit 34; actin related protein 2/3 complex subunit 2 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-EMKTITGKTFSSR |
| Molecular Weight | 34.3 |
| Target | ARPC2 |
| Amino Acid Sequence | EMKTITGKTFSSR |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 34-35kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3kDa according to NP_005722.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.. IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml. |
| Gene Id | Human GeneIDs: 10109. Non-Human GeneIDs: 76709 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ., The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly., J Cell Biol. 1997 Jul 28;138(2):375-84., PMID: 9230079 |
| Supplier | Everest Biotech |
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