| Product Name | Goat anti-Arylsulfatase A Antibody |
|---|---|
| Synonyms | ARSA; arylsulfatase A; MLD; cerebroside-sulfatase; cerebroside 3-sulfatase |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-YDLSKDPGENYN |
| Molecular Weight | 53.6; 44.9 |
| Target | Arylsulfatase A |
| Amino Acid Sequence | YDLSKDPGENYN |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 60-65Da band observed in Mouse and Rat Testis lysates (calculated MW of 53.7kDa according to Mouse NP_033843.2 and Rat NP_001030105.2;). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with th. IHC: Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u |
| Gene Id | Human GeneIDs: 410. Non-Human GeneIDs: 11883 (mouse); 315222 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Sevin C, Benraiss A, Van Dam D, Bonnin D, Nagels G, Verot L, Laurendeau I, Vidaud M, Gieselmann V, Vanier M, De Deyn PP, Aubourg P, Cartier N. , Intracerebral adeno-associated virus-mediated gene transfer in rapidly progressive forms of metachromatic leukodystrophy., Hum Mol Genet. 2006 Jan 1;15(1):53-64. Epub 2005 Nov 25. , PMID: 16311251 |
| Supplier | Everest Biotech |
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