| Product Name | Goat anti-ATF6 (aa437-449) Antibody |
|---|---|
| Synonyms | ATF6; activating transcription factor 6; ATF6A; cAMP-dependent transcription factor ATF-6 alpha; cyclic AMP-dependent transcription factor ATF-6 alpha |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-NSYRYDHSVSNDK |
| Molecular Weight | 74.6 |
| Target | ATF6 (aa437-449) |
| Amino Acid Sequence | NSYRYDHSVSNDK |
| Reactivity | Bovine, Canine, Human, Porcine, Rat |
| Applications | ELISA, FC, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 75-80kDa band observed in lysates of cell lines HeLa and MCF7, and in HeLa and Jurkat nuclear cell lysates (calculated MW of 74.6kDa according to NP_031374.2). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: |
| Gene Id | Human GeneIDs: 22926. Non-Human GeneIDs: 304962 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC., Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes., The Journal of biological chemistry 2012 Jun 287 (27): 23035-45., PMID: 22577136 |
| Supplier | Everest Biotech |
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