| Product Name | Goat anti-B7-H4 Antibody |
|---|---|
| Synonyms | B7-H4; B7H4; B7S1; B7X; B7h.5; FLJ22418; PRO1291; RP11-229A19.4; VCTN1; T cell costimulatory molecule B7x; immune costimulatory protein; V-set domain containing T cell activation inhibitor 1 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SKGKGNANLEYK |
| Molecular Weight | 30.9; 20.7 |
| Target | B7-H4 |
| Amino Acid Sequence | SKGKGNANLEYK |
| Reactivity | Canine, Human, Mouse, Rat |
| Applications | ELISA, FC, IF, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 26+21kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26kDa and 21kDa band observed in Human Adrenal Gland lysates, and approx 27kDa in Human Pancreas lysates (calc. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recomme |
| Gene Id | Human GeneIDs: 79679. Non-Human GeneIDs: 242122 (mouse); 295322 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Krambeck AE, Thompson RH, Dong H, Lohse CM, Park ES, Kuntz SM, Leibovich BC, Blute ML, Cheville JC, Kwon ED., B7-H4 expression in renal cell carcinoma and tumor vasculature: associations with cancer progression and survival., Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10391-6. Epub 2006 Jun 23., PMID: 16798883 |
| Supplier | Everest Biotech |
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