| Product Name | Goat anti-Calnexin Antibody |
|---|---|
| Synonyms | CANX; calnexin; CNX; FLJ26570; IP90; P90; major histocompatibility complex class I antigen-binding protein p88 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SKTPELNLDQFHDKT |
| Molecular Weight | 67.6 |
| Target | Calnexin |
| Amino Acid Sequence | SKTPELNLDQFHDKT |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 90-100kDa band observed in Human Cerebellum and Rat Brain lysates and in lysates of cell lines LNCaP and U251 (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.3kDa according to Rat NP_742005.1). This molecular weight is routinely obs. IHC: Paraffin embedded Human Cortex. Recommended concentration: 6µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of U251 cells and in the endoplasmic reticulum and plasma membranes of LNCaP cells. Recommended concentration: 10µg/ml. |
| Gene Id | Human GeneIDs: 821. Non-Human GeneIDs: 12330 (mouse); 29144 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Rosser MF, Grove DE, Chen L, Cyr DM, , Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2., Molecular biology of the cell 2008 Nov 19 (11): 4570-9., PMID: 18716059 |
| Supplier | Everest Biotech |
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