Goat anti-Caspase 3 Antibody

Category: Antibodies
Catalog
EB07286
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Product Name Goat anti-Caspase 3 Antibody
Synonyms CASP3; caspase 3; caspase 3, apoptosis-related cysteine protease; HGNC:1504; caspase 3, apoptosis-related cysteine peptidase; CPP32; CPP32B; SCA-1; PARP cleavage protease; SREBP cleavage activity 1; Yama; apopain; cysteine protease CPP32; procaspase3
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-RDVSKEDHSKRS
Molecular Weight 31.6
Target Caspase 3
Amino Acid Sequence RDVSKEDHSKRS
Reactivity Canine, Human, Mouse, Rat
Applications ELISA, IF, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 32kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6kDa according to Human NP_004337.2 and 31.4kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65kDa was also consistently ob. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.
Gene Id Human GeneIDs: 836
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Liadis N, Murakami K, Eweida M, Elford AR, Sheu L, Gaisano HY, Hakem R, Ohashi PS, Woo M. , Caspase-3-dependent beta-cell apoptosis in the initiation of autoimmune diabetes mellitus., Mol Cell Biol. 2005 May;25(9):3620-9. , PMID: 15831467
Supplier Everest Biotech

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