Goat anti-CBX3 / HP1 Gamma Antibody

Category: Antibodies
Catalog
EB06959
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Product Name Goat anti-CBX3 / HP1 Gamma Antibody
Synonyms CBX3; HP1-GAMMA; chromobox homolog 3 (HP1 gamma homolog, Drosophila) ; HGNC:1553; HECH; HP1Hs-gamma ; HP1 gamma homolog; chromobox homolog 3; chromobox homolog 3 (Drosophila HP1 gamma); heterochromatin protein HP1 gamma; heterochromatin-like protein 1
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-EAFLNSQKAGKEKD
Molecular Weight 20.8
Target CBX3 / HP1 Gamma
Amino Acid Sequence EAFLNSQKAGKEKD
Reactivity Bovine, Canine, Human, Mouse, Rat
Applications ELISA, FC, IF, IHC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx. 20kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 µg/ml.. IHC: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration: 2-3µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Gene Id Human GeneIDs: 11335. Non-Human GeneIDs: 12417 (mouse); 297093 (rat);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Vakoc CR, Mandat SA, Olenchock BA, Blobel GA. , Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin., Mol Cell. 2005 Aug 5;19(3):381-391. , PMID: 16061184
Supplier Everest Biotech

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