| Product Name | Goat anti-Centromere protein F / mitosin Antibody |
|---|---|
| Synonyms | CENPF; centromere protein F (350/400kD, mitosin); CENF; PRO1779; mitosin; AH antigen; CENP-F kinetochore protein; centromere protein F (400kD); cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400ka (mitosin); RP11-262H5.1; hcp-1; cent |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | GLESKGSENCKVQ |
| Molecular Weight | 358 |
| Target | Centromere protein F / mitosin |
| Amino Acid Sequence | GLESKGSENCKVQ |
| Reactivity | Bovine, Human |
| Applications | ELISA, IHC |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration.. IHC: Paraffin embedded Human Kidney. Recommended concentration: 3.75µg/ml. |
| Gene Id | Human GeneIDs: 1063 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Liao H, Winkfein RJ, Mack G, Rattner JB, Yen TJ. , CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis., J Cell Biol. 1995 Aug;130(3):507-18., PMID: 7542657 |
| Supplier | Everest Biotech |
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