| Product Name | Goat anti-CGI58 / ABHD5 Antibody |
|---|---|
| Synonyms | ABHD5; CGI58 protein; aCGI58; bhydrolase domain containing 5; CDS; IECN2; MGC8731; NCIE2 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-FPERPDLADQDR |
| Molecular Weight | 39.1 |
| Target | CGI58 / ABHD5 |
| Amino Acid Sequence | FPERPDLADQDR |
| Reactivity | Canine, Human, Mouse, Rat |
| Applications | ELISA, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:16000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 40kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1kDa according to NP_057090.2). Additional faint bands of 26kDa and 18kDa were also observed, and were successfully blocked by incubation with the immunizing peptide. Rec. IHC: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10µg/ml. |
| Gene Id | Human GeneIDs: 51099. Non-Human GeneIDs: 67469 (mouse); 316122 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R. , Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome., Cell Metab. 2006 May;3(5):309-19. , PMID: 16679289 |
| Supplier | Everest Biotech |
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