| Product Name | Goat anti-CHCHD3 Antibody |
|---|---|
| Synonyms | CHCHD3; coiled-coil-helix-coiled-coil-helix domain containing 3; FLJ20420 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | KFKRYESHPVCAD |
| Molecular Weight | 26.2 |
| Target | CHCHD3 |
| Amino Acid Sequence | KFKRYESHPVCAD |
| Reactivity | Bovine, Canine, Human, Mouse, Rat, Sheep |
| Applications | ELISA, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:16000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 26kDa band observed in Human Brain (Cerebellum) and Heart lysates (calculated MW of 26.2kDa according to NP_060282.1). Recommended concentration: 1-3µg/ml. A customer found ~30kDa in THP1 and in Human primary monocyte derived dendritic cells.. IHC: Paraffin embedded Human Heart and Skeletal Muscle. Recommended concentration: 5µg/ml. |
| Gene Id | Human GeneIDs: 54927. Non-Human GeneIDs: 296966 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Xie,J., Marusich,M.F., Souda,P., Whitelegge,J. and Capaldi,R.A., The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix. Coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11, FEBS Lett. 581 (18), 3545-3549 (2007), PMID: 17624330 |
| Supplier | Everest Biotech |
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