Goat anti-COX2 / PTGS2 Antibody

Category: Antibodies
Catalog
EB05286
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Product Name Goat anti-COX2 / PTGS2 Antibody
Synonyms PTGS2; COX2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; prostaglandin G/H synthase and cyclooxygenase; GRIPGHS; cyclooxygenase 2b
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-NPTVLLKERSTEL
Molecular Weight 69
Target COX2 / PTGS2
Amino Acid Sequence NPTVLLKERSTEL
Reactivity Bovine, Canine, Human, Porcine
Applications ELISA, FC, IF, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 75-80kDa band observed in lysates of cell lines A549 and Daudi (predicted MW of 69kDa according to NP_000954.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at roo. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of HepG2 and NIH3T3 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommende
Gene Id Human GeneIDs: 5743
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Ballif BA, Mincek NV, Barratt JT, Wilson ML, Simmons DL., Interaction of cyclooxygenases with an apoptosis- and autoimmunity associated protein., Proc Natl Acad Sci U S A. 1996 May 28;93(11):5544-9., PMID: 8643612
Supplier Everest Biotech

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