Goat anti-creatine kinase M-type Antibody

Category: Antibodies
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EB11996
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Product Name Goat anti-creatine kinase M-type Antibody
Synonyms CKM; creatine kinase, muscle; CKMM; M-CK; creatine kinase M chain; creatine kinase M-type; creatine kinase-M
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen NTHNKFKLNYKPEE-C
Molecular Weight 43.1
Target creatine kinase M-type
Amino Acid Sequence NTHNKFKLNYKPEE
Reactivity Canine, Human, Mouse
Applications ELISA, IHC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:128000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 45kDa band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kDa according to NP_001815.2). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.
Gene Id Human GeneIDs: 1158
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Diguet N, Mallat Y, Ladouce R, Clodic G, Prola A, Tritsch E, Blanc J, Larcher JC, Delcayre C, Samuel JL, Friguet B, Bolbach G, Li Z, Mericskay M., Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomyopathy., J Biol Chem. 2011 Oct 7;286(40):35007-19., PMID: 21768101
Supplier Everest Biotech

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