| Product Name | Goat anti-CSNK1E Antibody |
|---|---|
| Synonyms | CSNK1E; casein kinase 1, epsilon; RP1-5O6.1; HCKIE; MGC10398 ; CKI-epsilon; OTTHUMP00000063258; OTTHUMP00000063263; casein kinase 1 epsilon; casein kinase I epsilon |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-PASQTSVPFDHLGK |
| Molecular Weight | 47.3 |
| Target | CSNK1E |
| Amino Acid Sequence | PASQTSVPFDHLGK |
| Reactivity | Canine, Human, Mouse, Rat |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 48kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45kDa in nuclear lysates of cell line K562 (calculated MW of 47.3kDa according to NP_001276841.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at roo. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: |
| Gene Id | Human GeneIDs: 1454. Non-Human GeneIDs: 27373 (mouse); 58822 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Hammerlein A, Weiske J, Huber O. , A second protein kinase CK1-mediated step negatively regulates Wnt signalling by disrupting the lymphocyte enhancer factor-1/beta-catenin complex., Cell Mol Life Sci. 2005 Mar;62(5):606-18. , PMID: 15747065 |
| Supplier | Everest Biotech |
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