Goat anti-Cytochrome b reductase 1 Antibody

Category: Antibodies
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EB06633
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Product Name Goat anti-Cytochrome b reductase 1 Antibody
Synonyms CYBRD1; DCYTB; FLJ23462; cytochrome b reductase 1; duodenal cytochrome b; FRRS3; ferric-chelate reductase 3
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen CRNLALDEAGQRSTM
Molecular Weight 31.6
Target Cytochrome b reductase 1
Amino Acid Sequence CRNLALDEAGQRSTM
Reactivity Bovine, Canine, Human, Porcine
Applications ELISA, IHC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:16000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Preliminary experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1µg/ml antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfully used in WB on. IHC: In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5µg/ml. Paraffin embedded Human Small Intestine. Recommended concentration:
Gene Id Human GeneIDs: 79901
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Atanasova BD, Li AC, Bjarnason I, Tzatchev KN, Simpson RJ. , Duodenal ascorbate and ferric reductase in human iron deficiency., Am J Clin Nutr. 2005 Jan;81(1):130-3. , PMID: 15640471
Supplier Everest Biotech

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