| Product Name | Goat anti-Dynactin Antibody |
|---|---|
| Synonyms | DCTN1; dynactin 1 (p150, glued homolog, Drosophila); P135; DP-150; DAP-150; p150-glued; p150, Glued (Drosophila) homolog; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, Glued (Drosophila) homolog); HMN7B; OTTHUMP00000202491; dynactin 1; p150, Gl |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-QEQLHQLHSRLIS |
| Molecular Weight | 142; 127; 139; 127, 136, 140 |
| Target | Dynactin |
| Amino Acid Sequence | QEQLHQLHSRLIS |
| Reactivity | Human, Mouse |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:16000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx. 150kDa band observed in HeLa and MCF7 cell lysates, and in preliminary testing of HEK293 and Mouse Brain lysate (calculated MW of 142kDa according to NP_004073.2). Recommended concentration 0.3-1µg/ml. Primary incubation 1 hour at room temperatur. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-7µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally the nucleus of U2OS cells.. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeL |
| Gene Id | Human GeneIDs: 1639. Non-Human GeneIDs: 13191 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Holzbaur EL, Tokito MK. , Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization., Genomics. 1996 Feb 1;31(3):398-9. No abstract available., PMID: 8838327 |
| Supplier | Everest Biotech |
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