Goat anti-E2-230K / UBE2O Antibody

Category: Antibodies
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EB05761
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Product Name Goat anti-E2-230K / UBE2O Antibody
Synonyms UBE2O; ubiquitin-conjugating enzyme E2O; E2-230K; FLJ12878; KIAA1734
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen ALLEAGMPECTEDK
Molecular Weight 141
Target E2-230K / UBE2O
Amino Acid Sequence ALLEAGMPECTEDK
Reactivity Bovine, Canine, Human, Mouse, Porcine, Rat
Applications ELISA, IHC
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reporte. IHC: In paraffin embedded Human Heart shows textured staining in some myocardial fibres. Recommended concentration, 4-6µg/ml.
Gene Id Human GeneIDs: 63893. Non-Human GeneIDs: 217342 (mouse);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Yokota T, Nagai H, Harada H, Mine N, Terada Y, Fujiwara H, Yabe A, Miyazaki K, Emi M. , Identification, tissue expression, and chromosomal position of a novel gene encoding human ubiquitin-conjugating enzyme E2-230k., Gene. 2001 Apr 4;267(1):95-100., PMID: 11311559
Supplier Everest Biotech

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