Goat anti-EEA1 (aa821-835) Antibody

Category: Antibodies
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EB11689
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Product Name Goat anti-EEA1 (aa821-835) Antibody
Synonyms EEA1; early endosome antigen 1; MST105; MSTP105; ZFYVE2; early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-QETKIQHEELNNRIQ
Molecular Weight 162
Target EEA1 (aa821-835)
Amino Acid Sequence QETKIQHEELNNRIQ
Reactivity Human
Applications ELISA, IF, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:128000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 200kDa band observed in lysates of cell line HEK293 (calculated MW of 162kDa according to NP_003557.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubatiion 1 hour at room temperat. Notes: Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5µg/ml.
Gene Id Human GeneIDs: 8411
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Mishra A, Eathiraj S, Corvera S, Lambright DG., Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1)., Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10866-71., PMID: 20534488
Supplier Everest Biotech

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