| Product Name | Goat anti-ELMO2 Antibody |
|---|---|
| Synonyms | ELMO2; engulfment and cell motility 2 (ced-12 homolog, C. elegans); CED12; FLJ11656; KIAA1834; ced-12 homolog 2; PH domain protein CED12A; engulfment and cell motility 2; engulfment and cell motility 2; RP11-394O2.2; CED-12; ELMO-2 |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-PKEPSSYDFVYHYG |
| Molecular Weight | 82.6; 72.8 |
| Target | ELMO2 |
| Amino Acid Sequence | PKEPSSYDFVYHYG |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 80kDa band observed in lysates of cell lines HEK293, Jurkat, RAW264.7 and in Human Cerebellum and Rat Brain lysates (calculated MW of 82.6kDa according to Human NP_573403.1 and NP_877496.1 and Mouse NP_525026.2, and 83.7kDa according tp Rat NP_001. IHC: In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration: 3-6µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in A549 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. |
| Gene Id | Human GeneIDs: 63916. Non-Human GeneIDs: 140579 (mouse); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS., CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration., Cell. 2001 Oct 5;107(1):27-41., PMID: 11595183 |
| Supplier | Everest Biotech |
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