| Product Name | Goat anti-EndoPDI / TXNDC5 Antibody |
|---|---|
| Synonyms | TXNDC5; EndoPDI; thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide; thioredoxin domain containing 5 (endoplasmic reticulum) isomerase; Hcc-2; PDIA15; endothelial protei |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-SLHRFVLSQAKDEL |
| Molecular Weight | 47.6; 36.2 |
| Target | EndoPDI / TXNDC5 |
| Amino Acid Sequence | SLHRFVLSQAKDEL |
| Reactivity | Human |
| Applications | ELISA, FC, IF, IHC, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:64000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Approx 50kDa band observed in lysates of cell lines HEK293, A549, HeLa and HepG2 (calculated MW of 47.6kDa according to NP_110437.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-0.1µg/ml. Primary incubatio. IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of U2OS and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended con |
| Gene Id | Human GeneIDs: 81567 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Sullivan DC, Huminiecki L, Moore JW, Boyle JJ, Poulsom R, Creamer D, Barker J, Bicknell R., EndoPDI, a novel protein-disulfide isomerase-like protein that is preferentially expressed in endothelial cells acts as a stress survival factor., J Biol Chem. 2003 Nov 21;278(47):47079-88. Epub 2003 Sep 08., PMID: 12963716 |
| Supplier | Everest Biotech |
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