| Product Name | Goat anti-ERK3 / MAPK6 Antibody |
|---|---|
| Synonyms | MAPK6; mitogen-activated protein kinase 6; ERK3; HsT17250; PRKM6; p97MAPK; ERK-3; MAP kinase 6; MAP kinase isoform p97; MAPK 6; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; p97-MAPK; protein kinase, mitogen-activate |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | C-HSPVGSPLKSIQ |
| Molecular Weight | 82.7 |
| Target | ERK3 / MAPK6 |
| Amino Acid Sequence | HSPVGSPLKSIQ |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Applications | ELISA, IHC, IP, WB |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:128000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band . IHC: Paraffin embedded Human Heart. Recommended concentration: 10µg/ml.. Notes: Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were |
| Gene Id | Human GeneIDs: 5597. Non-Human GeneIDs: 50772 (mouse); 58840 (rat); |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Brand F, Schumacher S, Kant S, Menon MB, Simon R, Turgeon B, Britsch S, Meloche S, Gaestel M, Kotlyarov A., The extracellular signal-regulated kinase 3 (mitogen-activated protein kinase 6 [MAPK6])-MAPK-activated protein kinase 5 signaling complex regulates septin function and dendrite morphology., Mol Cell Biol. 2012 Jul;32(13):2467-78., PMID: 22508986 |
| Supplier | Everest Biotech |
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