Goat anti-Farnesoid X receptor / FXR Antibody

Category: Antibodies
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EB07540
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Product Name Goat anti-Farnesoid X receptor / FXR Antibody
Synonyms farnesoid X receptor; HRR1; NR1H4; nuclear receptor subfamily 1, group H, member 4 ; BAR; FXR; HRR-1; RIP14 ; farnesol receptor HRR-1
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen KSCREKTELTPDQQ
Molecular Weight 54.4; 54.8; 56.0; 55.4; 48.8
Target Farnesoid X receptor / FXR
Amino Acid Sequence KSCREKTELTPDQQ
Reactivity Bovine, Human
Applications ELISA, FC, IF
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:32000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Preliminary testing showed a band at approx 55kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5µg/ml antibody staining (calculated MW of 54.4kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration
Gene Id Human GeneIDs: 9971
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Huang W, Ma K, Zhang J, Qatanani M, Cuvillier J, Liu J, Dong B, Huang X, Moore DD. , Nuclear receptor-dependent bile acid signaling is required for normal liver regeneration., Science. 2006 Apr 14;312(5771):233-6. , PMID: 16614213
Supplier Everest Biotech

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